SH-2
ACC 643
Cell line:
SH-2
DSMZ no.:
ACC 643
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 2005 from the bone marrow of a 35-year-old Chinese man with AML-M2 after idarubicin/cysteine arabinoside treatment; cells were described in the literature to express natural killer cell-related antigens in addition to myeloid ones and to exhibit TP53 gene alteration; cells are tumorigenic in nude and SCID mice
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% Iscove's MDM + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; we recommend to start culture in a 12- or 24-well plate; optimal split ratio of 1:2 twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80 hours
Harvest:
maximum densitiy of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD56 +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 8% polyploidy; 57(54-58)<2n>X, -Y, +1, +2, +3, +4, +6, -11, +12, +13, +15, +18, +19, +22, +2mar, del(1)(q31.2q42)x2, der(4)t(1;4)(p22;q32), der(16)t(16;17)(q24;q12); related to published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list