DOGUM

human (Homo sapiens)

Burkitt lymphoma

established in 2003 from the pleural fluid of a 49-year-old woman with B-cell non-Hodgkin lymphoma (transformed follicular centroblastic) after failed combination chemotherapy and radiation

1

A

Transfer to third parties needs to be negotiated with the depositor.

lymphoid cells growing in large clumps in suspension; image

80% RPMI 1640 + 20% h.i. FBS

seed out at ca. 0.3-0.5 x 10⁶ cells/ml in 24-well-plates; maintain at ca. 0.7-1.5 x 10⁶ cells/ml; optimal split ratio of 1:2 to 1:4 every 2-5 days; after thawing cells may need a few days to recover

at 37 °C with 5% CO₂

ca. 50 hours; but up to 100 hours after recovery from N₂

maximum densitiy of ca. 4.5 x 10⁶ cells/ml

frozen with 70% medium, 20% FBS, 10% DMSO

negative in microbiological culture, PCR assays

CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

highly rearranged human hypodiploid karyotype with 6% polyploidy; 43(40-45)<2n>X, -X, -14, -15, der(1)t(1;14)(q32;q21.2), t(14;18)(q32;q21), t(2;8)(q14;q24), der(2)t(2;8), dup(4)(p12p15), add(5)(q13), add(6)(p23), der(6;17)(p1?0;q1?0), add(10)(p12), del(12)(q21), inv(13)(p12q21), der(15)t(5;15)(q14;q21), add(15)(p11)add(15)(q2?), der(18)t(14;18)(q32;q21), add(22)(p11), +2mar; carries t(14;18) effecting IGH-BCL2 with non-IG, MYC rearrangement present in markers; published karyotype not available

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

Print data sheet