SHI-1
ACC 645
Cell line:
SHI-1
DSMZ no.:
ACC 645
Species:
human (Homo sapiens)
Cell type:
acute myelocytic leukemia
Origin:
established in 2002 from the bone marrow of a 37-year-old male with relapsed acute monocytic leukemia (AML-M5b); described to carry a TP53 point mutation; carries a t(6;11)(q27;q23) and corresponding KMT2A-AFDN (MLL-MLLT4; MLL-AF6) fusion gene
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
round cells growing singly in suspension; image
Medium:
80-90% Iscove's MDM + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:4 to 1:10 every 2-4 days; maintain at 0.3-1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40-60 hours
Harvest:
maximum densitiy of ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD13 +, CD15 +, CD19 -, CD33 +, CD34 -, CD235a -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype with 12% polyploidy; 46(44-46)<2n>XY, -18, +mar, t(6;11)(q27;q23), add(8)(p23), del(10)(p11), del(17)(p12); carries t(6;11) effecting rearrangement of KMT2A (MLL) with AFDN (MLLT4; AF6); matches published karyotype
Molec. Genetics:
expression of fusion gene KMT2A-AFDN (MLL-MLLT4; MLL-AF6) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list