DND-39
ACC 648
Cell line:
DND-39
DSMZ no.:
ACC 648
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the pleural effusion of a 28-year-old man with American-type EBV-negative Burkitt lymphoma in 1977
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round to polygonal cells, growing singly or in small clusters in suspension; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml, optimal split ratio of 1:3 every 2-3 days, maintain at ca. 0.5-1 x 10 6 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-50 hours
Harvest:
maximum densitiy of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotetraploid karyotype with 4% polyploidy; 86(83-89)<4n>XXYY, -2, -4, -10, -10, -12, -14, -16, -16, +22, +22, der(5)t(2;5)(p24;q14)x2, del(6)(q15)x2 , t(8;14)(q24;q32)x2, add(14)(q32), der(18)add(18)(q21)x2; carries t(8;14) effecting IGH-MYC rearrangement; no reference karyotype available
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list