HCEC-H9C1
ACC 649
Cell line:
HCEC-H9C1
DSMZ no.:
ACC 649
Species:
human (Homo sapiens)
Cell type:
corneal endothelium
Origin:
HCEC-H9C1 is a clonal subpopulation started in 2005 from the parental cell line HCEC-12 (ACC 646) established from normal cells of the posterior epithelium of the cornea of a 91-year-old woman transformed with the early region of the SV40 genome including genes encoding large T-antigen and small t-antigen; the subclone is described in the literature to represent developing or transitional cells of the so-called corneal endothelium
Biosafety level:
1, GMO-S1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
polygonal, weakly adherent initially after plating, cells proliferate also in floating aggregates; cells do not form confluent monolayers
Medium:
Human-Endothelial-SFM + 10 ng/ml FGF-2 (observe the shelf-life of the medium; do not apply h.i. FBS in order to keep differentiation status)
Subculture:
seed out at ca. 0.5 x 106 cells/25 cm2 flask; flasks must be coated with 10 µg/ml laminin and 10 mg/ml chondroitin sulfate; split culture 1:2 to 1:3 every 3-4 days using trypsin/EDTA (max. 2 min); stop trypsination by using medium containing protease inhibitor cocktail (Sigma P1860) at 500-fold dilution; do not apply antibiotics, cells are highly sensitive to antibiotics (cytotoxic)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of ca. 4-5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line HCEC-12
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list