WILL-1
ACC 651
Cell line:
WILL-1
DSMZ no.:
ACC 651
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 2007 from the bone marrow mononuclear cells of an 82-year-old man with diffuse large B-cell lymphoma in the leukemic phase
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at 0.7-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-5 days; maintain at 0.3-1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 30-50 hours; but 50-70 hours after recovery from liquid nitrogen
Harvest:
maximum densitiy of ca. 3.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +,CD80 +, CD138 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human bimodal hypodiploid/hyperdiploid karyotype; 45(41-46)<2n>X, -Y, add(1)(q24), der(2)t(1;2)(q12;q3?7)inv(1)(q2?4q4?1), i(4q), der(6)t(6;8)(q27;q24), t(8;14)(q24;q32), der(17)t(X;17)(q23;p13), i(21q); sdl with idem, 57(50-58)<2n>X, -Y, +5, +6, +7, +8, +9, +11, +13, +der(14), +20, +21; carries t(8;14) effecting IGH/MYC rearrangement confirmed by FISH; non-IGH-MYC rearrangement present in der(6); resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list