UPCI-SCC-114
ACC 662
Cell line:
UPCI-SCC-114
DSMZ no.:
ACC 662
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established from an oral squamous cell carcinoma of a 71-year-old man; described in the literature to exhibit a point mutation in exon 7 (R248Q) of the tumor suppressor gene p53
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004).
DSMZ Cell Culture Data:
Morphology:
epithelial cells growing adherently as monolayer
Medium:
80-90% MEM (with Earle's salts) + 10-20% h.i. FBS + 2 mM L-glutamine + non-essential amino acids
Subculture:
seed out at ca. 1 x 106 cells/25 cm2 flask; split confluent culture 1:3 every 3-4 days using trypsin/EDTA for more than 10 minutes (prior to using trypsin, cells should be washed twice with PBS)
Incubation:
at 37 °C with CO2
Doubling time:
ca. 60 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list