LAN-2
ACC 671
Cell line:
LAN-2
DSMZ no.:
ACC 671
Species:
human (Homo sapiens)
Cell type:
neuroblastoma
Origin:
established in 1977 from the primary tumor of a 3-year-old girl with neuroblastoma (clinical Stage IV)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
40% RPMI 1640 + 40% MEM (with Earle's salts) + 20% h.i. FBS + 2 mM L-glutamine + non-essential amino acids
Subculture:
seed out at 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA; (cells might grow slowly after thawing); washing of cells with PBS may be recommended to reduce occuring debris
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
cell harvest of ca. 55 x 106 cells/175 cm2
Storage:
frozen with 35% RPMI 1640, 35% MEM (with Earle's salts), 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list