NALM-16
ACC 680
Cell line:
NALM-16
DSMZ no.:
ACC 680
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established from the peripheral blood of a 12-year-old girl with acute lymphoblastic leukemia (ALL) at relapse in 1977; cells are described in the literature to carry a near haploid karyotype
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml in a 12- or 24-well plate and initially with 20% FBS; maintain at ca. 1-1.5 x 106 cells/ml; split saturated culture 1:2-3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36 hours
Harvest:
maximum density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Plasmocin, then negative in microbiological culture, PCR assays
Immunology:
CD3-, CD10(+), CD13-, CD19-, CD20(+), CD34-,
CD37(+), CD38+, CD80-, CD138+, HLA-DR(+),
sIgG-, sIgM-, sIg-kappa-, sIg-lambda-, cIgG-,
cIgM-, cIg-kappa-, cIg-lambda-, cyCD79a+; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 52(49-53)<2n>XX, +14, +14, +18, +18, +21, +21, del(X)(q13);
clonally related to published near haploid karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list