NALM-20
ACC 681
Cell line:
NALM-20
DSMZ no.:
ACC 681
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established in 1989 from the peripheral blood of a 62-year-old man at diagnosis of acute undifferentiated leukemia (AUL); ; cells were described in the literature to carry a Philadelphia chromosome t(9;22) with a BCR-ABL1 e1-a2 fusion gene; the cell line is a serial sister cell line of NALM-21 (ACC 682)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.8-1.5 x 106 cells/ml in 24-well plates; maintain at ca. 0.6-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days; cells grow slowly after recovery from liquid nitrogen
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
cell harvest of ca. 2.5-3.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 +, CD19 +, CD38 +, cyCD79a -, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 48(45-48)<2n>XY, +8, +8, inv(4)(p14q35), del(8)(p12)x2, t(9;22)(q34;q11); carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list