BL-100
ACC 684
Cell line:
BL-100
DSMZ no.:
ACC 684
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the bone marrow of a woman with EBV-negative Burkitt lymphoma with translocation t(8;22) after EBV infection in culture
Reference(s):
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
mostly single round cells in suspension, partly in small clusters; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-2.0 x 106 cells/ml; split saturated culture 1:3 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum densitiy of ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture and continuously in PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 47(44-48)<2n>XX, +12, del(1)(q23), der(3)t(1;3)(q23;q2?2), t(8;22)(q24;q11); carries t(8:22) effecting rearrangement of IGL with MYC; matches unpublished karyotypic data
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, SMRV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list