Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 1990 from the bone marrow of a 22-year-old man with acute myeloid leukemia (AML M2) in second relapse. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round to polymorphic cells growing singly and in small clusters in suspension;
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Medium:
90% RPMI 1640 + 10% h.i. FBS + 10 ng/ml GM-CSF
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days; alternatively to GM-CSF, 5-10% conditioned medium of the cell line 5637 may be used. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 35-50 hours
Harvest:
maximum density at ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +;
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
clonally heterogeneous human near-diploid karyotype; 46(42-48)<2n>X, -X, +5, +6, +16, add(2)(q22), i(5)(p10), add(6)(q27) or i(6)(p10), der(7)t(7;9)(q35.2;q21), t(8;21)(q22;q22), ider(9)(p10)add(9)(p21), add(11)(p15) or inv(11)(p15q24), der(16;17)(q10;q10), der(17;19)(p10;q10); resembles published karyotype; carries t(8;21) effecting RUNX1-RUNX1T1 (AML1-ETO) rearrangement
Molec. Genetics:
expression of fusion gene RUNX1-RUNX1T1 (AML1-ETO) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
