UCSD-AML1
ACC 691
Cell line:
UCSD-AML1
DSMZ no.:
ACC 691
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the bone marrow of a 73-year-old woman with acute myeloid leukemia at relapse in 1989; cells were described in the literature to be cytokine-dependent (GM-CSF, IL-6, or M-CSF) and to carry the translocations t(3;3)(q21;q26) and t(12;22)(p13;q12) leading to MECOM (EVI1) overexpression and to ETV6-MN1 (TEL-MN1) fusion gene, respectively
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml GM-CSF
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split culture 1:3 to 1:5 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximum density at ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hypodiploid karyotype with 10% polyploidy; 45(42-45)<2n>XX,-7, t(3;3)(q21;q26), t(12;22)(p13;q12); carries t(3;3) and t(12;22) effecting MN1 and ETV6/MN1 rearrangements, respectively; precisely matches published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list