Leibniz Institute DSMZ: Details

Cell line:

OCI-LY18

DSMZ no.:

ACC 699

Species:

human (Homo sapiens)

Cell type:

B cell lymphoma

Origin:

established in 1988 from the pleural fluid of a 56-year-old man with diffuse large B cell lymphoma (DLBCL, stage IIIB, at relapse); cells were described in the literature to be EBV negative and to carry a three-way translocation t(8;18;14) leading to alterations of MYC, BCL2, and IGH genes

Reference(s):

14427, 16050, 16051

Biosafety level:

1

Permissions and restrictions:

A

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

small round cells growing singly in suspension; image; image; image

Medium:

90% RPMI 1640 + 10% h.i. FBS

Subculture:

seed out at ca. 0.5 x 10⁶ cells/ml; maintain at ca. 0.5-3.0 x 10⁶ cells/ml; split culture 1:3-1:5 every 2-3 days

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 30-40 hours

Harvest:

maximum density at ca. 3.5 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in microbiological culture, PCR assays

Immunology:

CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:

human hyperdiploid karyotype with 4% polyploidy; 50(48-51)<2n>X, -Y, +9, +17, +18, +18, +18, der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), del(9)(q21.1), dup(12)(q11q15), der(14)t(14;18)(q32;q21), der(17)t(17;18)(p11.2;q21)t(18;14)(q21;q32), der(18)t(14;18)(q32;q21)x2, der(18)t(14;18)(q32;q21)t(14;8)(q32;q24)x2; carries complex series of two break IGH rearrangements involving MYC and BCL2 on ders(8)/(14)/(17)/(18); resembles published karyotype

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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