GRANTA-452
ACC 713
Cell line:
GRANTA-452
DSMZ no.:
ACC 713
Species:
human (Homo sapiens)
Cell type:
B cell leukemia
Origin:
derived from a case of B cell acute lymphoblastic leukemia (B-ALL) following transformation from follicular B cell non-Hodgkin lymphoma (B-NHL); described in the literature to carry a t(2;9) (p1;p13) and a t(9;14;18) (p13;q32;q21), to exhibit a biallelic deletion of CDKN2A as well as a BCL2 MBR rearrangement
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round cells growing singly in suspension; image
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1 x 106 cells/ml; split culture 1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximum density at ca. 2-3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype with 5% polyploidy; 49(46-50)<2n>XXY, +X, +7, +11, der(1)t(1;7)(p36;p15), der(2)t(2;4)(p21;p15.2), der(3)t(1;3)(p26;q11), der(4)t(4;6)(p11;q22), del(6)(q13), trp(8)(q22q24), t(8;22)(q24;q11), der(9)t(2;9)(p13;p15.2), der(9)t(9;14)(p13;q22)t(14;18)(q32;q21), der(14)t(9;14)(p21;q22), t(16;19)(p13;p13), der(18)t(14;18)(q32;q21), der(20)t(9;20)(p21;p13)ins(9;?)(p21.2;?); carries IGH/BCL2 and IGL/MYC rearrangements, together with a complex series of rearrangements effecting deletion of CDKN2 (homozygous) and PAX5 (hemizygous); consistent with published karyotype data
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list