LAZ-221
ACC 716
Cell line:
LAZ-221
DSMZ no.:
ACC 716
Species:
human (Homo sapiens)
Cell type:
B cell precursor leukemia
Origin:
established in 1977 from the peripheral blood of a 24-year-old woman with "null cell" acute lymphoblastic leukemia (null-ALL) at diagnosis
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1.5 x 106 cells/ml in 24-well plates; cells grow slowly during the first week, do not split cell culture and only exchange spent medium partially during the first week; maintain at ca. 1-2 x 106 cells/ml; split culture 1:2 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-80 hours
Harvest:
maximum density at ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 -, CD5 +, CD7 -, CD8 -, CD10 +, CD13 -, CD19 +, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR +, TCRalpha/beta -, TCRgamma/delta -, sm/cyIgG -, sm/cyIgM +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypodiploid karyotype; 45(41-46)<2n>XX, -12, der(2)t(2;18)(q36;q21), der(9)t(9;12)(p12;q11), del(18)(q22); resembles published karyotype
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list