MEG-A2
ACC 718
Cell line:
MEG-A2
DSMZ no.:
ACC 718
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia
Origin:
established 1995 from the peripheral blood of a 24-year-old man with chronic myelogenous leukemia (CML) in megakaryoblastic crisis after chemotherapy; cells are described in the literature to display several megakaryocytic features and to be inducible to megakaryocytic differentiation; cells carry the Philadelphia chromosome with an e14-a2 (b3-a2) BCR-ABL1 fusion gene
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% Iscove's MDM + 20% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; cells may grow slowly in the first week after thawing; split saturated culture 1:2 to 1:3 every 3-4 days; maintain at ca. 0.5-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 40 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD19 -, CD33 +, cyCD68 +, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human flat-moded hyperdiploid/hypotriploid karyotype; 51-70<2n>XY, +3, +5, +8, +8, +8, +11, +13, +18, +19, -20, +21, +21, del(8)(q12)x2, der(8)t(8;20)(q12;q13)x2, der(9)t(9;22)(q34;q11), del(12)(p13), der(19)t(4;19)(q21;p13), der(19)t(19;20)(q13;p12), del(21)(q22.2)x2, der(22)t(9,22)x1-3; carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list