Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia
Origin:
established 1995 from the pleural effusion of a 30-year-old man with chronic myelogenous leukemia (CML) in T cell lineage blast crisis after bone marrow transplantation; cells carry the Philadelphia chromosome with a e14-a2 (b3-a2) BCR-ABL1 fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 every 3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-72 hours
Harvest:
maximum density at ca. 2.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 -, CD3 -, CD5 +, CD6 +, CD7 +, CD10 -, CD13 -, CD14 -, CD19 -, CD20 -, CD34 -, cyCD68 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; 48(46-50)<2n>XY, +22, +22, del(1)(q24), del(6)(q21q24), dup(7)(p1?4q3?5)inv(7)(p14q35), t(9;22)(q34;q11), add(21)(p11), der(22)?inv(22)(p11q11)t(9;22)(q34;q11)x2; carries complex t(9;22) effecting BCR-ABL1 rearrangement with 3x genomic amplification in der(22); matches published karyotype
Molec. Genetics:
expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
