MHH-TALL-2
ACC 728
Cell line:
MHH-TALL-2
DSMZ no.:
ACC 728
Species:
human (Homo sapiens)
Cell type:
T cell acute lymphoblastic leukemia
Origin:
established in 1994 from the peripheral blood of a boy with T cell acute lymphoblastic leukemia (T-ALL)
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
single cells growing in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1-1.5 x 106 cells/ml in 24-well plates; maintain at 1-3 x 106 cells/ml; split saturated culture 1:2 or 1:3 every 3-4 days; cells are difficult to culture; possibly no cell growth during the first week after thawing; a significant amount of cell debris may be present all the time
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-72 hours
Harvest:
maximum density at ca. 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta +, TCRgamma/delta -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human diploid karyotype with 2% polyploidy; 46(44-46)<2n>XY; no consistent abnormality detected
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list