HT-93
ACC 735
Cell line:
HT-93
DSMZ no.:
ACC 735
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 1993 from the peripheral blood of a 66-year-old man with acute promyelocytic leukemia (AML FAB M3) at relapse; described in the literature to carry t(15;17) and t(1;12) leading to PML-RARA and ETV6-ABL2 (ETV6-ARG) fusion genes, respectively
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out and maintain at ca. 0.5-1.0 x 106 cells/ml; optimal split ratio 1:2 every 2-3 days; culture should be started with 20% FBS in 24-well-plates
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative PCR assay
Immunology:
CD3 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype; 46(44-46)<2n>XY, t(1;12)(q25;p13), der(2)t(1;2)(q25;q31), der(4)t(1;4)(q21;q25)t(1;12)(q25;p13), der(6)del(6)(p23)t(4;6)(q25;q23), t(15;17)(q22;q21); carries complex t(1;12) and t(15;17) rearrangements effecting ETV6-ABL2 (ETV6-ARG) and PML-RARA rearrangements, respectively; resembles published karyotyp
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list