human (Homo sapiens)
established from the nephrectomy specimen of a large lobulated Wilms´ tumor of a 4-year-old girl; cells were described in the literature to express NCAM, SALL1, and CITED1, and to contain a TP53 mutation
Permissions and restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
80% Dulbecco´s MEM (with 4.5 g/l glucose) + 20% h.i. FBS
seed out at ca. 0.5-1 x 106 cells/25 cm2 flask; cells grow slowly and may need 1-2 days for adherence to the plastic surface; split confluent culture 1:2 once a week using trypsin/EDTA (wash cells twice with PBS before adding trypsin/EDTA)
at 37 °C with 5% CO2
ca. 1 week
cell harvest of ca. 10 x 106 cells/80 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay
To inquire about expression of EpCAM and intermediate filaments, contact firstname.lastname@example.org.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV +
|Frozen culture||450,- €|
|Growing culture |
(please inquire for exact delivery time)
|DNA isolated from cell line (25 µg)||560,- €|
|DNA isolated from cell line (5 µg)||140,- €|
see price list