BA-D5
ACC 75
Cell line:
BA-D5
DSMZ no.:
ACC 75
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-rat fiber/myosin)
Origin:
this hybridoma cell line was described in the literature to secrete a mouse monoclonal antibody (IgG) that reacts with rat type 1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain) in western blotting; the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin from bovine atrium
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM (4.5 g/L glucose) + 10% h.i. FBS (or horse serum) + 2 mM L-glutamine
Subculture:
split saturated culture at 1:5 every 2-3 days; optimal density at about 0.5 x 106 cells/ml; after thawing, culture first with 20% FBS, viability may drop to as low as 50-60%
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25 hours
Harvest:
cell harvest of about 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
secretion of a IgG2b monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine hypertetraploid/pentaploid karyotype with 10% polyploidy - 90(88-100)<4n> - 1-3 centric fusion markers and 2 dmin present in most cells
Viruses:
ELISA: reverse transcriptase negative; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list