Species:
human (Homo sapiens)
Cell type:
chronic B cell leukemia
Origin:
established in 1998 from leukemic B-cells of a 70-year-old man with chronic lymphocytic leukemia at diagnosis (CLL, Rai stage II). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and
RNA-Seq)
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Morphology:
round cells growing partly in clumps in suspension and slightly adherent;
image;
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Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.2 - 0.5 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-60 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in PCR assay
Immunology:
CD3-, CD10-, CD13-, CD19(+), CD20, CD33-, CD34-, CD37+, CD38+, CD80+, CD138(+), HLA-DR+, sIgkappa-, sIglambda+, cIgG-, cIgM+, cIgkappa-, cIgkappa+;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human near-diploid karyotype; 46(44-46)<2n>XY, der(10)t(10;11)(q26;q24.1), del(13)(q12q32); carries del(13) associated with CLL; marginal resemblance to original karyotype
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
