D-11 - Discontinued
ACC 77
Cell line:
D-11 - Discontinued
DSMZ no.:
ACC 77
Species:
fish - rainbow trout (Oncorhynchus tshawytscha)
Cell type:
liver
Origin:
Caution: D-11 is a derivative of fish cell line R1.
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
adherent, fibroblastoid cells growing as monolayers
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS
Subculture:
split confluent culture 1:2 every 7-10 days using trypsin/EDTA in PBS with Ca2+/Mg2+ at room temperature for 5-10 min
Incubation:
at room temperature (about 20 °C) with CO2, cells can be stored temporarily (up to 1 week) at 4 °C
Doubling time:
about 80 hours or more
Harvest:
about 5-15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization and PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Oncorhynchus tshawytscha
Viruses:
PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list