BLN-3
ACC 787
Cell line:
BLN-3
DSMZ no.:
ACC 787
Species:
human (Homo sapiens)
Cell type:
glioblastoma
Origin:
established in 2013 from an intraoperative glioblastoma specimen
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
most cells grow adherently like neuronal cells in monolayer, some cells grow loosely attached in clumps
Medium:
Neurobasal medium + 0.5x B-27 supplement with vitamin A, 0.5x N-2 supplement, 20 ng/ml bFGF, 20 ng/ml EGF, 2 mM L-glutamine; omit FBS!
Subculture:
seed out at ca. 1.0 x 106 cells in an 80 cm2 flask; cells grow slowly after thawing; split culture 1:5 to 1:10 every 5-8 days with trypsin/EDTA (cells can be detached by tapping the flask or by pipetting)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2-4 days
Harvest:
cell harvest of ca. 10 x 106 cells/175 cm2
Storage:
frozen with 90% medium, 10% DMSO (no FBS added)
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list