Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1996 from the pleural effusion of a 52-year-old man with diffuse large B cell lymphoma (DLBCL). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
Morphology:
round cells growing singly or in small clumps in suspension;
image;
image;
image
Medium:
85-90% RPMI 1640 + 10-15% h.i. FBS
Subculture:
seed out at ca. 0.1-0.3 x 106 cells/ml; optimal split ratio 1:5 - 1:10 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-30 hours
Harvest:
maximum density at ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hyperdiploid karyotype; -47(43-48)<2n>XY, +7, der(8)del(8)(p22.2)t(8:14)(q24;q32), der(9)t(2;9)(p25:p21), der(14)t(8;14)(q24;q32), der(17)del(17)(p11.2)del(17)(q21q22); sdl with del(9)(q11); carries t(8;14) effecting IGH-MYC rearrangement; der(17) carries 1q21-derived focal amplicon inserted at 17p11 effecting MCL1 activation
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
| Delivery form | | Prices |
|
| Frozen culture | | 450,- € |
Growing culture
(please inquire for exact delivery time) | | 900,- € |
| DNA isolated from cell line (25 µg) | | 560,- € |
| DNA isolated from cell line (5 µg) | | 140,- € |
see price list
