MBU-TS4
ACC 801
Cell line:
MBU-TS4
DSMZ no.:
ACC 801
Species:
human (Homo sapiens)
Cell type:
keratinocytes
Origin:
established in 2011 from primary keratinocytes of a neonatal foreskin; cells were transduced with retroviral vectors containing human telomerase, SV40 early region, and epitope-tagged mutated HRAS(V12) linked to IRES-driven GFP expression; MBU-TS4 is a xenograft-derived side line of MBU-T in Swiss nude mice
Reference(s):
Biosafety level:
2, GMO-S2
Risk assessment:
The primary keratinocyte cells were transduced with retroviral vectors containing a) human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential and is assigned to biosafety level 2.
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract
Subculture:
seed out at ca. 1 x 106 cells/175 cm2 flask; split 1:3 - 1:4 every 6-8 days using Accutase (rinse two times with PBS and incubate for about 15 min at 37°C)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 80-100 hours
Harvest:
cell harvest of ca. 3-4 x 106 cells/175 cm2
Storage:
frozen with 90% Keratinocyte SFM, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list