UPCI-SCC-036

human (Homo sapiens)

squamous cell carcinoma

established in 1993 from a new, poorly differentiated primary tumor of the tonsils (grade 2,T3N1) of a 56-year-old man with head and neck squamous cell carcinoma. Described to carry a chromosomal 11q13 amplification and several other aberrations. The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-414-648-4004) at the University of Pittsburgh, Office of Technology Management

15061, 14735, 15176

1

Originally, the tumor cells were described to be HPV-11 positive. However, the accessioned cell line was tested negative for HPV.

A

The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004).

epithelioid cells growing adherently as monolayer; image; image

90% MEM (with Earle's salts) + 10% h.i. FBS + non-essential amino acids + 2 mM L-glutamine

seed out at about 1 x 10⁶ cells/80 cm²; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA (incubate up to 10 min with trypsin)

at 37 °C with 5% CO₂

ca. 48 hours

cell harvest of ca. 3-5 x 10⁶ cells/80 cm² flask

frozen with 70% medium, 20% FBS, 10% DMSO

contamination was eliminated with Plasmocin, then negative in PCR asay

to make inquiries about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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