Leibniz Institute DSMZ: Details

Cell line:

OVPA-8

DSMZ no.:

ACC 871

Species:

human (Homo sapiens)

Cell type:

ovarian cancer

Origin:

established in 2011 from the ascites fluid of a 47-year-old woman with recurrent high-grade serous ovarian cancer after three different chemotherapies (diagnosed in 2005 with stage III C papillary serous adenocarcinoma G3). Cells are described to exhibit multiple chromosomal aberrations, TP53 and BRCA1 mutations and the loss of one copy of BRCA2. Also described to be resistant to cisplatin and AZD4547, but sensitive to paclitaxel and CPL304-110-01

Reference(s):

18203

Biosafety level:

1

Permissions and restrictions:

A

Depositor-specific restrictions:

Transfer to third parties needs to be negotiated with the depositor.

DSMZ Cell Culture Data:

Morphology:

epithelioid cells growing adherently as monolayer, carrying many intracellular dark granules; image; image

Medium:

80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine

Subculture:

seed out at about 1 x 10⁶ cells/80 cm²; split subconfluent (70-80%) culture 1:5 - 1:10 once or twice a week using trypsin/EDTA

Incubation:

at 37 °C with 5% CO₂

Doubling time:

50-60 hours

Harvest:

cell harvest of ca. 10 x 10⁶ cells/80 cm² flask

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Immunology:

to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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