HEK293T-GIGYF1/2-null
ACC 874
Cell line:
HEK293T-GIGYF1/2-null
DSMZ no.:
ACC 874
Species:
human (Homo sapiens)
Cell type:
embryonal kidney
Origin:
this cell line is part of a set of cell line models carrying mutations in different genes affecting mRNA processing and translation efficiency (ACC 872 - ACC 876).
Single clone derivative of 293T (DSMZ ACC 635) which was modified by CRISPR/CAS9 editing.
In this cell line the ORFs of both genes, GIGYF1 and GIGYF2, were disrupted by several deletions and insertions.
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
(see also parental 293T, DSMZ ACC 635)
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + 2mM L-glutamine
Subculture:
seed out at about 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-4 days by tapping the flask (trypsin/EDTA is not necessary)
Incubation:
at 37 °C with 5-10% CO2
Doubling time:
ca. 30-40 hours
Harvest:
cell harvest of ca. 5 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
the disruptions of the GIGYF genes were verified by sequence analysis as described in Ref 18207 (Sgromo et al 2018). For internal control the following primers can be used : D-GIGYF1-ex7F: AAGTCAGGGAGAAGAAGAGTGGGTAC, D-GIGYF1-ex7R: AGTCAGCAAGGCGGGATGGAG;
D-GIGYF1-ex16F: GAAGAGTTAATTGGTATGTCCCAAAGACTG, D-GIGYF1-ex16R: CGTGCGTGCGAGCCCTCA; D-GIGYF2-F: CCTCCTTACCCCAGATTAACATAGAAAG, D-GIGYF2-R: TATAAACCACCTAGCACCTGTCAAATACT.
Absence of the GIGYF proteins were confirmed by Western blot.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list