UPCI-SCC-084
ACC 878
Cell line:
UPCI-SCC-084
DSMZ no.:
ACC 878
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established from a recurrent squamous cell carcinoma (moderately differentiated, stage T2N2b) of the oral retromolar trigone of
a 52-year-old man; cells are described to carry a chromosomal 11q13 amplification and a frameshift mutation within the TP53 gene.
The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-412-648-4004) at the University of Pittsburgh, Office of Technology Management
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004).
DSMZ Cell Culture Data:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:5 - 1:10 once or twice a week using trypsin/EDTA for more than 10 minutes
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 22 hours
Harvest:
cell harvest of ca. 10-15 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to make inquiries about expression of EpCAM and intermediate filaments, please contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list