UPCI-SCC-103
ACC 879
Cell line:
UPCI-SCC-103
DSMZ no.:
ACC 879
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established from the primary tumor of the tongue of a 27-year-old woman with squamous cell carcinoma (well differentiated, stage T1N0); cells are described to carry a chromosomal 11q13 amplification, loss of the protein kinase ATM gene and a mutation in the TP53 gene. The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-412-648-4004) at the University of Pittsburgh, Office of Technology Management
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004).
DSMZ Cell Culture Data:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + non-essential amino acids
Subculture:
seed out at 1-2 x 106 cells/80 cm2; split confluent culture 1:5 - 1:10 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25-30 hours
Harvest:
cell harvest of ca. 5-8 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
mutation in the TP53 (Arg 306) was verfied by sequencing
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list