THB-5
ACC 88
Cell line:
THB-5
DSMZ no.:
ACC 88
Species:
murine (mouse) (Mus musculus)
Cell type:
hybridoma (anti-C3d receptor and anti-EBV receptor)
Origin:
established by fusion of the P3X63AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with human B cell line SB; cells were reported to produce a monoclonal antibody (IgG2akappa) reacting with the human C3d receptor (CD21) and an EBV receptor of 145 kDa
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
85% RPMI 1640 + 15% h.i. FBS
Subculture:
optimal density at 0.5 x 106 cells/ml, minimal density at 0.1 x 106 cells/ml; split 1:3 to 1:5; after thawing, it is advised to use feeder cells for the first 7-10 days; seed out at ca. 0.5-1.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
maximal density at 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
secretion of a IgG2a monoclonal antibody
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list