RTgill-W1
ACC 899
Cell line:
RTgill-W1
DSMZ no.:
ACC 899
Species:
fish -rainbow trout (Oncorhynchus mykiss)
Cell type:
gill
Origin:
established in 1990 from the gill of a juvenile rainbow trout; cells are used in OECD guideline Test No. 249 (Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay) for testing of acute toxicity of chemicals; cells are further described to
support growth of certain viruses and fish parasites
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
For approval of distribution to for-profit organizations please contact kristin.schirmer@eawag.ch
DSMZ Cell Culture Data:
Medium:
95% Leibovitz´ L15 (without phenol red) + 5% (not inactivated) FBS
Subculture:
cells grow very slowly; seed out 1-1.5 x 106 cells in a 25 cm2 flask; split confluent culture 1:2 every 10-14 days as follows: rinse 2x with 1ml versene (0.2 g/l) add 0.6-0.8 ml trypsin (0.25% (for 1-2 min)) and detach carefully; avoid sheering the cells or formation of foam; change medium every 5-7 days
Incubation:
at ca. 20°C in the dark, without CO2 gas exchange
Doubling time:
ca. 10-12 days
Harvest:
cell harvest of 8-10 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with MRA and BM-cyclin by the originator, then proofed as negative by PCR at the DSMZ
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Oncorhynchus mykiss species.
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list