SU-DHL-2
ACC 902
Cell line:
SU-DHL-2
DSMZ no.:
ACC 902
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1973 from the pleural effusion of a 73-year-old woman with diffuse histiocytic lymphoma (DHL) in relapse and after chemotherapy with prednisone and chlorambucil. Recently cell line was assigned as ABC-DLBCL subtype (activated B cell-like diffuse large B cell lymphoma)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 0.3 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; maintain up to 0.8 x 106 cells/ml but adjust to 0.1 -0.2 x 106 cells/ml before the weekend
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 24 hours
Harvest:
maximal density of 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD20 +, CD34 -, CD37 +, CD38 -, CD80 +, CD138 -, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list