SU-DHL-7
ACC 903
Cell line:
SU-DHL-7
DSMZ no.:
ACC 903
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 1974 from the pleural effusion of a 47-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), described at that time as diffuse histiocytic lymphoma (DHL), denominated today as diffuse large B-cell lymphoma (DLBCL)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; maintain between 0.5 and 1.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
maximal density of 2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD2 -, CD3 -, CD4 -, CD8 -, CD10 +, CD13 -, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, cyIgG +, cyIgM -, cykappa -, cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
expression of fusion gene IGH-BCL2 confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list