BL-65
ACC 920
Cell line:
BL-65
DSMZ no.:
ACC 920
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1982 from a 3-year-old girl with Burkitt lymphoma; cells are EBV-positive and were described to carry a t(8;14) translocation; a matched B-lymphoblastoid cell line is available (IARC-305, DSMZ ACC 928)
Reference(s):
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
mostly round cells growing singly, partly in aggregates in suspension; image
Medium:
85-90% Iscove's MDM + 10-15% h.i. FBS + 100 µM Thioglycerol
Subculture:
seed out at about 0.5 x 106 cells/ml; split
1:4 to 1:10 every 2-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-40 hours
Harvest:
maximal density of ca. 1.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD30 -, CD34 -, CD37 +, CD38 +, cy CD79a +, CD138 -, HLA-DR +, cy IgM +, cy IgG -, cy kappa -, cy lambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching autologous IARC-305
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list