CCLF_MELM_0026_T_B
ACC 935
Cell line:
CCLF_MELM_0026_T_B
DSMZ no.:
ACC 935
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established in 2021 from a brain metastasis of a 58-year old woman with untreated malignant melanoma; a sister cell line, CCLF_MELM_0026_T_A, exists
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
AR5 medium after SOP CELL301-AR5 but without antibiotics
Subculture:
seed out at about 0.5 x 106 cells in a T75 flask; split at about 80% confluency 1:2 to 1:3 every 3-6 days (rinse culture 2x with PBS and use prewarmed TrypLE Express)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3 days
Harvest:
maximum density of ca. 1 x 106 cells/T25 flask
Storage:
frozen with RecoveryTM Cell Culture Freezing Medium
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Molec. Genetics:
BRAF K601E homozygous, IL7R V138I homozygous, TP53 T211I homozygous; data according to gene panel analysis at CCLF, verified by RNA-seq analysis at DSMZ
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 900,- € | |
| Growing culture (please inquire for exact delivery time) | 1800,- € | |
| DNA isolated from cell line (25 µg) | 1120,- € | |
| DNA isolated from cell line (5 µg) | 280,- € | |
see price list