CCLF_HNSC_0004_T
ACC 938
Cell line:
CCLF_HNSC_0004_T
DSMZ no.:
ACC 938
Species:
human (Homo sapiens)
Cell type:
squamous cell carcinoma
Origin:
established in 2016 from a patient derived xenograft model (PDX) of the primary tumor of head and neck squamous cell carcinoma of a 78-year old male; pretreatment unknown
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
95% RETM + 5% FBS + 25 ng/ml Cholera toxin, after CELL310-RETM but without antibiotics
Subculture:
seed out 0.1-0.5 x 106 cells in a T75 flask, split culture at 80-90% confluence 1:3-1:7 every 3-7 days using Accutase
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 20-30 hours
Harvest:
maximum density of ca. 6-7 x 106 cells/T75 flask
Storage:
frozen with RecoveryTM Cell Culture Freezing Medium
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list