CCLF_NEURO_0107_T
ACC 940
Cell line:
CCLF_NEURO_0107_T
DSMZ no.:
ACC 940
Species:
human (Homo sapiens)
Cell type:
glioblastoma
Origin:
established in 2017 from a biopsy of a recurrent tumor in the brain of a 55-year-old woman with glioblastoma;
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
the cells are cultivated serum-free in NSA medium after SOP CELL307-NSA but without antibiotics
Subculture:
use laminin-coated flasks and seed out at about 0.5 x 106 cells in a T25 flask or 1 x 106 cells in a T75 flask; split confluent culture 1:2 every 3-5 days using warm TrypLE Express, after rinsing the cell layer with PBS; change medium every 3 - 5 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 4 days
Harvest:
maximum density of ca. 2 x 106 cells/T25 flask
Storage:
frozen with RecoveryTM Cell Culture Freezing Medium
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Molec. Genetics:
RB1 L389*homozygous, TP53 R237H homozygous; data according to gene panel analysis at CCLF, verified by RNA-seq analysis at DSMZ
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 900,- € | |
| Growing culture (please inquire for exact delivery time) | 1800,- € | |
| DNA isolated from cell line (25 µg) | 1120,- € | |
| DNA isolated from cell line (5 µg) | 280,- € | |
see price list