KIS-1
ACC 947
Cell line:
KIS-1
DSMZ no.:
ACC 947
Species:
human (Homo sapiens)
Cell type:
diffuse large B-cell lymphoma
Origin:
established in 1985 from ascites fluid of a 53-year-old man with B-cell lymphoma (diffuse large B-cell lymphoma, DLBCL); cells are positive for CD30 (alias name KI-1); a translocation t(9;14)(p13;q32) affecting PAX5 is described. Karyotype analysis is provided in the original paper
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml;
split culture 1:2 to 1:6 every 2-3 days; maintain at ca. 0.1-0.9 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 24-48 hours
Harvest:
cell harvest of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 +, CD30 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, CD80 -
; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list