YK-M2
ACC 949
Cell line:
YK-M2
DSMZ no.:
ACC 949
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established in 1985 from the peripheral blood of a 31-year-old man with acute monoblastic leukemia pretreated with chemotherapy and irradiation. Cells are described as near triploid, expressing Fc-gamma receptors and differentiation could be induced by vitamine D
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 1 x 106 cells/ml; maintain at 0.8 - 1.5 x 106 cells/ml and split carefully (approx. 1:2 every 2-3 days)
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50 hours
Harvest:
maximal density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR +, cy IgG+, cy IgM -, cy kappa (+), cy Iambda -, cy CD3 -, cy CD68 +, cy CD79a -;
image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list