BL-31
ACC 951
Cell line:
BL-31
DSMZ no.:
ACC 951
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established before 1982 from lymphoma cells of the bone marrow of a 14-year-old boy diagnosed with sporadic Burkitt Lymphoma in relapse; cells are EBV-negative and were described to carry t(8;14) translocation
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at about 0.5 x 106 cells/ml in a 24-well plate; split 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Harvest:
maximal density of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 -, CD138 +, HLA-DR +, sm IgG +, smI gM +, sm/cy kappa +, sm/cy lambda -, cyCD79a (+), cy IgG -, cy IgM +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list