BL-33
ACC 952
Cell line:
BL-33
DSMZ no.:
ACC 952
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1981 from bone marrow of a 35-year-old man with EBV-negative Burkitt Lymphoma (BL) in leukemic phase after treatment. The patient has a history with a gastric tumor. Cells are described to carry t(8;22) involving MYC
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Subculture:
cells grow fast; seed out at about 0.5 x 106 cells/ml; split 1:3 to 1:10 every 2-3 days, maintain at 0.2-2 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 24 hours
Harvest:
max density of 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 +, HLA-DR +, sm IgG -, sm IgM +, sm kappa -, sm/cy lambda +, cy IgM +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list