BL-60
ACC 953
Cell line:
BL-60
DSMZ no.:
ACC 953
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1984 from the maxillary biopsy of a 4-year-old girl with EBV-positive Burkitt lymphoma; described to carry a t(8;22) chromosomal translocation
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
max density of 3 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 (+), CD138 +, HLA-DR +, sfIgG +, sfIgM +, sfIgKappa -, sfIgLambda +, cyIgG -, cyIgM +, cyIgKappa +, cyIgLambda +, cyCD79a (+); image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list