BL-89
ACC 954
Cell line:
BL-89
DSMZ no.:
ACC 954
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1984 from a bone marrow sample of a 6-year old boy with EBV-positive Burkitt lymphoma; described to carry a c-Myc-IgL translocation t(8:22) for one allele, in additio, mutations in exon 1 of c-MYC for the other allele
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% Iscove's MDM + 20% h.i. FBS + 100 µM alpha-thioglycerol + 2mM L-glutamine
Subculture:
seed out at about 0.5 x 106 cells/ml; in the first 3-5 days after thawing, cells do not grow and viability may decrease; later, split only 1:2 and exchange medium every 2-3 days, maintain at 0.4-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 3 days
Harvest:
max density of 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD37 +, CD38 +, CD80 -, CD138 -, sfIgG +, sfIgM -, sfIgKappa +, sfIgLambda +, cyCD79a +, cy IgG +, cyIgM -, cyIgLambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list