SL-15
ACC 955
Cell line:
SL-15
DSMZ no.:
ACC 955
Species:
human (Homo sapiens)
Cell type:
splenic lymphoma
Origin:
EBV-immortalized cell line established in 2003 from peripheral blood of a 53-year-old man with splenic lymphoma with villous lymphocytes (SLVL, leukemic form of splenic marginal zone lymphoma). The patient has a history of hepatitis B virus-associated hepatitis followed by a liver transplantation; cells are described to carry t(9;14)(p13;q32) affecting PAX5; the karyotype is provided in the original publication
Reference(s):
Biosafety level:
1
Risk assessment:
The cell line is positive for EBV (HHV-4, strain of B95-8 cell line) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) EBV positive cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects.
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at about 0.5 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days, maintain at 0.1-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-40 hours
Harvest:
max density of 1.4 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD5 -, CD10 -, CD13 -, CD19 +, CD20 +, CD25 (+), CD34 -, CD37 +, CD38 +/-, CD80 +, CD138 +/-, HLA-DR +, sIgG +, sIgM -, sIg-kappa +, cIgG +, cIgM -, cIg-kappa +, cIg-lambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV + (cells carry the EBV strain B95.8), HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list