Maska-98
ACC 956
Cell line:
Maska-98
DSMZ no.:
ACC 956
Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established in 1998 from the bone marrow of a 61-year-old woman with aggressive multiple myeloma; described to produce high level of LDH
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 0.5-1 x 106 cells/ml; split 1:2 every 2-3 days, maintain at about 0.8 x 106 cells/ml; culture does not reach viabilty higher than 80%; arising debris may mimick a bacterial contamination
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
max density of 1-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3-, CD10-, CD13-, CD19-, CD20-, CD34-, CD37-, CD38+, CD80-, CD138+, HLA-DR-, sIgG+, sIgM-, sIg-kappa-, sIg-lambda+, cIgG+, cIgM-, cIg-kappa-, cIg-lambda+, cyCD79a-; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/-2 -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list