MUG-CC1
ACC 957
Cell line:
MUG-CC1
DSMZ no.:
ACC 957
Species:
human (Homo sapiens)
Cell type:
clival chordoma
Origin:
established in 2014 from a 72-year old man with clival chordoma (classic subtype of the cranial base); cells are described to carry gains of several chromosomes
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
Iscove's MDM + RPMI 1640 4:1 + 10% h.i. FBS + 20 ng/ml testosterone + 1% ITS (insuline, transferrin, sodium selenite) + 2 mM L-glutamine
Subculture:
seed out at about 1 x 106 cells in a T25 flask; split at 80% confluency 1:2 every 6-10 days using trypsin/EDTA or Accutase; it is recommended to collect possibly floating cells by centrifugation
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 7 days
Harvest:
maximal density of ca. 10 x 106 cells in a T175 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV -, XMLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list