Leibniz Institute DSMZ: Details

Cell line:

MUG-Lucifer-prim

DSMZ no.:

ACC 958

Species:

human (Homo sapiens)

Cell type:

clear cell sarcoma

Origin:

established in 2019 from the primary tumor of a 23-year-old woman with clear cell sarcoma of the knee joint; sister cell line of the metastasic tumor is also available (MUG-Lucifer-met ACC 959)

Reference(s):

40814

Biosafety level:

1

Permissions and restrictions:

A

DSMZ Cell Culture Data:

Morphology:

small, round, polygonal and spindle-shaped cells growing in suspension and adherently; semi-adherent, about 50-80% of cells are in suspension, 10-20% are loosely adherent, 10-20% are adherent; image; image; image

Medium:

90% DMEM/F12 with Glutamax + 10% h.i. FBS

Subculture:

seed out at 0.4-0.8 x 10⁵ cells/cm²; split culture 1:6-1:10 every 5 days; it is important to collect all (suspended and adherent) cells, use trypsin/EDTA

Incubation:

at 37 °C with 10% CO₂

Doubling time:

about 30-40 hours

Harvest:

0.3-0.6 x 10⁶ cells/cm²

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to sister cell line MUG-Lucifer-met

Molec. Genetics:

fusion gene EWSR1::ATF1 was verified by RT-PCR; Reported whole or partial chromosome gains of chromosomes 5, 7, 8, and 17, and partial losses of chromosomes 9 and 15 were confirmed by SNP-array analysis. In contrast to MUG-Lucifer-met, no partial gain of chromosome 1 was detected.

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV -1/2 -, XMLV -,

Supplied as:

Delivery form		Prices

Frozen culture		450,- €
Growing culture (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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